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簡(jiǎn)要描述:青旗(上海)生物技術(shù)發(fā)展有限公司,總部位于上海浦東新區(qū),依托本地高校資源,逐步發(fā)展成為以生物技術(shù)為主的研發(fā)、生產(chǎn)、培訓(xùn)為一體的綜合化產(chǎn)業(yè)平臺(tái),在標(biāo)準(zhǔn)化細(xì)胞庫(kù)建立及細(xì)胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營(yíng)原代細(xì)胞、細(xì)胞系、ELISA試劑盒、感受態(tài)細(xì)胞和HPLC檢測(cè)等科研產(chǎn)品與服務(wù)。我們秉承對(duì)用戶負(fù)責(zé)的態(tài)度,以對(duì)科研的高度嚴(yán)謹(jǐn),以嚴(yán)格的質(zhì)量控制,為廣大生物醫(yī)學(xué)科研用戶提供更優(yōu)質(zhì)的服務(wù)!
更新時(shí)間:2021-05-26
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品牌 | 其他品牌 | 貨號(hào) | BFN60806666 |
---|---|---|---|
規(guī)格 | T25培養(yǎng)瓶x1 1.5ml凍存管x2 | 供貨周期 | 現(xiàn)貨 |
主要用途 | 僅供科研 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè) |
細(xì)胞名稱 | 人食管上皮細(xì)胞HET-1A | ||
貨物編碼 | BFN60806666 | ||
產(chǎn)品規(guī)格 | T25培養(yǎng)瓶x1 | 1.5ml凍存管x2 | |
細(xì)胞數(shù)量 | 1x10^6 | 1x10^6 | |
保存溫度 | 37℃ | -198℃ | |
運(yùn)輸方式 | 常溫保溫運(yùn)輸 | 干冰運(yùn)輸 | |
安全等級(jí) | 1 | ||
用途限制 | 僅供科研 1類 |
培養(yǎng)體系 | DMEM高糖+10%FBS+1%雙抗 | ||
培養(yǎng)溫度 | 37℃ | 二氧化碳濃度 | 5% |
簡(jiǎn)介 | 人食管上皮細(xì)胞HET-1A取自25歲男性。 | ||
序列突變 | / | ||
注釋 | Doubling time: 27.4 +- 3.6 hours (PubMed=21127259). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [pRSV-T]. Omics: Deep RNAseq analysis. Omics: Transcriptome analysis. | ||
STR信息 | Amelogenin X,Y CSF1PO 10,12 D5S818 11,12 D7S820 9 D13S317 11 D16S539 9,11 TH01 7 TPOX 11 vWA 16 | ||
參考文獻(xiàn) | Patent=WO1991005062A1 Stoner G.D., Reddel R.R., Harris C.C. Human esophageal epithelial cell lines. Patent number WO1991005062A1, 18-Apr-1991
PubMed=1703038 Stoner G.D., Kaighn M.E., Reddel R.R., Resau J.H., Bowman D., Naito Z., Matsukura N., You M., Galati A.J., Harris C.C. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51:365-371(1991)
PubMed=8504475; DOI=10.1093/carcin/14.5.833 Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C. p53 mutations in human immortalized epithelial cell lines. Carcinogenesis 14:833-839(1993)
PubMed=20139130; DOI=10.1093/carcin/bgq036 Duggan S.P., Behan F.M., Kirca M., Smith S.M., Reynolds J.V., Long A., Kelleher D.P. An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels. Carcinogenesis 31:936-945(2010)
PubMed=20843300; DOI=10.1042/BC20100071 Underwood T.J., Derouet M.F., White M.J., Noble F., Moutasim K.A., Smith E., Drew P.A., Thomas G.J., Primrose J.N., Blaydes J.P. A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture. Biol. Cell 102:635-644(2010)
PubMed=21127259; DOI=10.1152/ajpgi.00461.2010 Goldman A., Chen H.D.R., Roesly H.B., Hill K.A., Tome M.E., Dvorak B., Bernstein H., Dvorak K. Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis. Am. J. Physiol. Gastrointest. Liver Physiol. 300:G292-G302(2011)
PubMed=28224081; DOI=10.1016/j.gdata.2017.02.002 Akagi I., Ishibashi O. RNA-Seq analysis of human cell lines established from normal and neoplastic esophageal squamous epithelium. Genomics Data 12:4-6(2017) |
青旗(上海)生物技術(shù)發(fā)展有限公司,總部位于上海浦東新區(qū),依托本地高校資源,逐步發(fā)展成為以生物技術(shù)為主的研發(fā)、生產(chǎn)、培訓(xùn)為一體的綜合化產(chǎn)業(yè)平臺(tái),在標(biāo)準(zhǔn)化細(xì)胞庫(kù)建立及細(xì)胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營(yíng)原代細(xì)胞、細(xì)胞系、ELISA試劑盒、感受態(tài)細(xì)胞和HPLC檢測(cè)等科研產(chǎn)品與服務(wù)。我們秉承對(duì)用戶負(fù)責(zé)的態(tài)度,以對(duì)科研的高度嚴(yán)謹(jǐn),以嚴(yán)格的質(zhì)量控制,為廣大生物醫(yī)學(xué)科研用戶提供更優(yōu)質(zhì)的服務(wù)!