細(xì)胞名稱(chēng)

人B淋巴細(xì)胞GM12878

貨物編碼

BFN60810358

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研   3類(lèi)

培養(yǎng)體系

90%DMEM(不含丙酮酸鈉）+10%FBS+1%三抗

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人B淋巴細(xì)胞GM12878取自女性外周血。

注釋

Part of: CEPH/Utah pedigree cell line collection.

Part of: ENCODE project common cell types; tier 1.

Part of: Genetic Testing Reference Material (GeT-RM) samples.

Part of: Genome in a Bottle (GIAB) consortium samples.

Part of: International Genome Sample Resource (1000 genomes project) cell lines.

Registration: CEPH Families Reference Panel; 146302.

Registration: National Institute of Standards and Technology, Standard Reference Materials; HG001.

Population: Utah residents with ancestry from Northern and Western Europe.

Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: DNA methylation analysis.

Omics: Deep RNAseq analysis.

Omics: Genome sequenced.

Omics: H3K27ac ChIP-seq epigenome analysis.

Omics: H3K27me3 ChIP-seq epigenome analysis.

Omics: H3K36me3 ChIP-seq epigenome analysis.

Omics: H3K4me1 ChIP-seq epigenome analysis.

Omics: H3K4me2 ChIP-seq epigenome analysis.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: H3K79me2 ChIP-seq epigenome analysis.

Omics: H3K9ac ChIP-seq epigenome analysis.

Omics: H3K9me3 ChIP-seq epigenome analysis.

Omics: H4K20me1 ChIP-seq epigenome analysis.

Omics: Mitochondrial genome sequenced.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Derived from sampling site: Peripheral blood.

基因突變

CYP2C19 p.Pro227Pro (c.681G>A); cryptic splice acceptor activation; allele CYP2C19*2 (Coriell).

HLA信息

/

STR信息

/

參考文獻(xiàn)

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Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing.

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Papp E., Hallberg D., Konecny G.E., Bruhm D.C., Adleff V., Noe M., Kagiampakis I., Palsgrove D., Conklin D., Kinose Y., White J.R., Press M.F., Drapkin R., Easwaran H., Baylin S.B., Slamon D., Velculescu V.E., Scharpf R.B.

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Cell Rep. 25:2617-2633(2018)

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Osorio D., Yu X., Yu P., Serpedin E., Cai J.J.

Single-cell RNA sequencing of a European and an African lymphoblastoid cell line.

Sci. Data 6:112-112(2019)

PubMed=31401124; DOI=10.1016/j.jmoldx.2019.06.007

Gaedigk A., Turner A., Everts R.E., Scott S.A., Aggarwal P., Broeckel U., McMillin G.A., Melis R., Boone E.C., Pratt V.M., Kalman L.V.

Characterization of reference materials for genetic testing of CYP2D6 alleles: a GeT-RM collaborative project.

J. Mol. Diagn. 21:1034-1052(2019)